Precision genome engineering technologies enable targeted, highly efficient alteration of DNA sequences in living cells or organisms. Given their customizable nature, these technologies promise to be broadly useful both as biomedical research tools and as novel therapeutics for gene-based diseases. Given the rapidity with which this field moves, it is challenging for both newcomers and experts alike to stay updated with all of the latest advances. Furthermore, genome engineering necessarily encompasses a broad range of scientific expertise that includes fundamental mechanisms of DNA repair, basic science studies of bacterial-derived systems that form the basis of many of these technologies, innovations with genome engineering tool platforms, a wide variety of research applications spanning from model organisms to plants and mammalian cells, and pre-clinical and clinical studies aimed at translation into human therapeutics. The goal of this Keystone Symposia meeting is to bring together scientists from academia and industry with diverse but relevant expertise in a setting conducive to discussion of new results and potential collaborative efforts. Invited talks will cover a wide range of topics ranging from fundamental basic science through to clinical translation studies. Attendees of this meeting will have the opportunity to hear about the latest findings in this fast-paced field and to establish collaborations with scientists who have complementary expertise.