Proteolytic enzymes (proteases) govern crucial intra- and extra-cellular function in all classes of life. Notably, the dysregulation of proteolysis or unwanted protease-related activity underpins the development and progression of major diseases that represent leading causes of death globally. Proteases and the molecules that control these enzymes are thus major targets for therapeutic intervention. The themes of our meeting will include understanding the role and control of proteases in cardiovascular disease, cancer and infectious diseases, amongst others. We will further explore the successes and challenges that surround the development of new generation therapeutics designed to control proteases and related systems. The meeting has a strong history in confronting controversies that surround mechanism. Crucially, many of the fundamental discoveries made in this regard now underpin major drug discovery programs. We will therefore explore some of the most perplexing questions in mechanism and biology that currently challenge protease biologists. Themes centering on mechanism will include protease inhibitors, deubiquitinating enzymes (DUBs), and proteolysis in and around the lipid membrane environment. We will also include a special focus on understanding proteases as they interact with the extracellular matrix and other specialised structures present in the extracellular environment. Finally, in our late breaking session we will highlight the latest and greatest discoveries that pertain to proteases from all walks of life. The meeting will include presentations by both established and younger scientists from around the world. The emphasis throughout the meeting will be on presenting unpublished cutting edge data. Further, through vibrant poster sessions, we will ensure all delegates have the opportunity to present their data for discussion and debate. As in 2012, we are delighted to highlight the associated GRS on Proteolytic Enzymes and Their Inhibitors. The GRS will highlight the work of graduate students and post-docs and will provide a wonderful opportunity to further explore the function and dysfunction of proteolytic systems.