The Smart Cities Symposium Prague 2025 aims on exchange of ideas and best practices in the field of Smart cities. It covers whole range of topics, from the system point of view, through data mining and data processing, smart grids, up to multi-agent systems and other soft computing approaches. The objective of this conference is to define the future applications for the field of smart cities. The results should be based on the theoretical backgrounds, but aim on usage of best practices for real world applications. The main themes of the SCSP 2025 will be cooperative cities not only in terms of sustainable mobility, but also the way to climate-neutral cities. We encourage researchers with different background to participate and share their findings. The Symposium aims to be a multidisciplinary forum for exchanging ideas and best practices in the field of Smart Cities not limited to theory but also including real world applications.