The rapid development and improvement of network education and multimedia technology has opened up a new way for the reform of higher vocational colleges teaching model and teaching method. To meet the need of modern education and teaching, based on the provincial and university level excellent course construction, we construct the basic framework of professional teaching resources library and collect, process, design and produce the multimedia courseware. We also record teaching video clips, establish test database. The application of network education technology and independent design and development of medical laboratory network teaching resources platform enrich and update the teaching content and enhance students' knowledge and ability, innovation ability. It also expands their learning space and plays an important role in their autonomous learning.