摘要详情
8 / 2025-03-24 15:39:48
Construction of a Fear Scoring System in Laying Hens and Its Association with Immune Status and Gut Microbiota
fear score, laying hens, open field test, immune response, Gut Microbes
摘要待审
Compared to the tonic immobility test (TI), commonly used to assess fear status, the open field test (OFT) provides a more comprehensive evaluation of fear levels in hens. Based on key fear-related indicators from previous studies, we developed a systematic fear scoring system and explored its association with immunity and gut microbiota. Ninety-six individually caged Lindian-hens (60 weeks old) underwent an OFT at 62 weeks and a TI at 64 weeks. Fear scores correlated positively with TI duration and negatively with vocalization and exploration, validating the system.
Hens from different fear score groups (1, 3, 5 points, n=6/group) were selected for serum analysis. The 5-point group compared with the 1-point showed significantly higher IL-1β, IL-6, TNF-α, and IgY, but lower IL-10 and IL-2(P < 0.05). 16S-rRNA sequencing revealed lower bacterial richness in the 5-point group, dominated by Firmicutes, while the 1-point group was enriched in Bacteroidetes.
Microbial α-diversity in the 1-point group was higher than 5-point while β-diversity revealed distinct microbial structures between the 1- and 5-point，with the 3-point group being intermediate. Gut immune analysis indicated increased IL-1β, IL-6, IFN-γ, and IgY mRNA in the 5-point group, whereas IL-4, IL-8, IL-10, and IgA were reduced(P < 0.05). Correlation analysis linked Bacteroides to IL-10 and IgA (positive) and IL-1β and IgY (negative), while Lactobacillus and Prevotella correlated with IL-6 and TNF-α, respectively(P < 0.05, |r| > 0.6）.
This study establishes a fear scoring system and highlights interactions between fear, immunity, and gut microbiota, offering insights into poultry welfare.
Hens from different fear score groups (1, 3, 5 points, n=6/group) were selected for serum analysis. The 5-point group compared with the 1-point showed significantly higher IL-1β, IL-6, TNF-α, and IgY, but lower IL-10 and IL-2(P < 0.05). 16S-rRNA sequencing revealed lower bacterial richness in the 5-point group, dominated by Firmicutes, while the 1-point group was enriched in Bacteroidetes.
Microbial α-diversity in the 1-point group was higher than 5-point while β-diversity revealed distinct microbial structures between the 1- and 5-point，with the 3-point group being intermediate. Gut immune analysis indicated increased IL-1β, IL-6, IFN-γ, and IgY mRNA in the 5-point group, whereas IL-4, IL-8, IL-10, and IgA were reduced(P < 0.05). Correlation analysis linked Bacteroides to IL-10 and IgA (positive) and IL-1β and IgY (negative), while Lactobacillus and Prevotella correlated with IL-6 and TNF-α, respectively(P < 0.05, |r| > 0.6）.
This study establishes a fear scoring system and highlights interactions between fear, immunity, and gut microbiota, offering insights into poultry welfare.
重要日期
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会议日期
10月20日
2025
至10月23日
2025
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04月15日 2025
摘要截稿日期
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05月01日 2025
摘要录用通知日期
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06月30日 2025
初稿截稿日期
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08月01日 2025
终稿截稿日期
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08月31日 2025
初稿录用通知日期
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10月23日 2025
注册截止日期
主办单位
International Research Center for Animal Environment and Welfare (IRCAEW)
Chinese Society of Agricultural Engineering (CSAE)
China Agricultural University (CAU)
Rongchang District People’s Government
The National Center of Technology Innovation for Pigs
Chinese Society of Agricultural Engineering (CSAE)
China Agricultural University (CAU)
Rongchang District People’s Government
The National Center of Technology Innovation for Pigs
承办单位
Chongqing Academy of Animal Sciences (CAAS)
Key Lab of Agricultural Engineering in Structure and Environment, Chinese Ministry of Agriculture, Beijing, China
Key Lab of Agricultural Engineering in Structure and Environment, Chinese Ministry of Agriculture, Beijing, China
联系方式
- Ms. Wen Liu
- 11******@qq.com
- +86********
历届会议
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2023年10月23日 中国 Chongqing
2023动物环境与福利化养殖国际研讨会 -
2021年10月21日 中国 Chongqing
2021动物环境与福利化养殖国际研讨会 -
2019年10月21日 中国
2019动物环境与福利化养殖国际研讨会 -
2017年10月23日 中国 Chongqing
2017畜禽健康环境与福利化养殖国际研讨会 -
2015年10月23日 中国 重庆市
2015 动物环境与福利国际研讨会 -
2015年10月23日 中国 重庆市
2015年动物环境国际研讨会 (ISAEW2015) -
2013年10月19日 中国 重庆市
畜禽健康环境与福利化养殖国际研讨会
