78 / 2023-06-30 09:44:03

Isolation, Identification and Degradation Characteristics of Functional Bacteria for NH3 and H2S Degradation

NH3 降解菌 ；H2S 降解菌 ；筛选 ；鉴定 ；优化

In order to reduce the emission of malodorous gas during the stacking of kitchen waste, two strains CN5 and CS2 with high degradation of NH₃ and H₂S were isolated and screened from kitchen waste by enrichment and domestication, qualitative primary screening and quantitative secondary screening methods. They were identified as Enterococcus sp. and Pseudomonas sp. by 16S rDNA sequence analysis. The optimal deodorizing conditions of microbial deodorant were determined by single factor experiment: culture temperature was 35℃, pH was 7, carbon nitrogen ratio was 25:1, carbon source was sucrose, nitrogen source was ammonium chloride, and the degradation rate of NH₃ and H₂S could be maintained at about 85%. Strains CN5 and CS2 grew best in the mixed state of 2:3, and the maximum value of OD₆₀₀ was 1.342. The determination of ammonia nitrogen content and sulfate content, it was found that the final ammonia nitrogen content was 50.5 mg/L, and the degradation rate of bacteria to ammonia nitrogen was 85.3%. The sulfate content was 302.7 mg/L, and the sulfate production rate of bacterial agent was 89.2%. The results showed that the two strains screened from the kitchen waste had good ability to degrading NH₃ and H₂S, and the two strains had synergistic effect with each other.