250 / 2024-08-26 15:29:37

Dual-Mode Scanning Two-Photon Microscopy System Based on Off-Axis Parabolic Mirror

Off-Axis Parabolic Mirror; Two-Photon Imaging; Dual-Mode Scanning; Confocal Relay; Aberrations

The imaging quality of a two-photon imaging system is enhanced by using a resonant scanner and two axial galvanometer scanners through two sets of off-axis parabolic mirrors relay. The x-axis galvanometer scanner is positioned at the focus of the second off-axis parabolic mirror in the first relay set and the first off-axis parabolic mirror in the second relay set. The system achieves dual-mode scanning by independently controlling the deflections of the resonant scanner and the x-axis galvanometer scanner. Located after the second set of off-axis parabolic mirrors is a confocal relay consisting of a scanning lens and a tube lens. The purpose of this relay is to achieve conjugate alignment of the y-axis galvanometer scanner target with the rear aperture of the objective lens.The simulation results show that this system improves field curvature and distortion compared with traditional doublet lens relay system, significantly reduces aberrations, and effectively enhances imaging quality. The first scanning mode achieves precise two-photon microscopy imaging with a field of view of 0.75 mm × 1 mm by controlling the resonant scanner and the y-axis galvanometer scanner. The second scanning mode, which controls the x-axis and y-axis galvanometer scanners, enables wide-field two-photon microscopy with a field of view of 2.5 mm × 2.5 mm.