The urban heat island (UHI) effect has been drawing more and more attention in the era of climate change. A lot of studies examined how urban can-yon geometry, as an important characteristic of urban form, influences the UHI effect. However, most of them assume empty urban canyons except for street trees, while in the real world there are often other physical elements inside the canyon. Parked vehicles are one of them, which are widely seen because of the widely adopted on-street parking policies in cities. Their geometry and material could change the energy balance in urban canyons. This study investigates how much on-street parking influences the UHI effect and human thermal comfort in urban streets. In this research, the microclimate conditions of streets in a typical residential neighborhood in Seoul during two summer days were simulated using ENVI-met 4.32 software and the results were validated with the field measured data. The outdoor human thermal comfort was further assessed as the PMV indicator using the ENVI-met. The influence of cars parked on-street was examined by the comparison of scenarios with street parking and without street parking. The results show that street parking scenario has higher air temperature, lower wind speed and lower relative humidity. For the human thermal comfort, it adds to heat stress levels in general, especially during the noon time. .The findings point out the importance of considering street parking in UHI mitigation studies in Seoul. The significant thermal influence of street parking can also provide an additional perspective for the parking policies in urban redevelopment projects in Seoul.