Based on the railway passenger traffic flow between cities, this paper captures the shortest time between the 338 prefecture-level administration units in the country by using the network data of China Railway Customer Service Center. This paper explores the spatial pattern of urban time accessibility and the spatial connections and hierarchical characteristics of the inter-city time accessibility through the network analysis and the spatial analysis of GIS. According to the time accessibility between cities, isotime circles and daily-communication-areas of municipalities and provincial capital cities are analyzed. And the urban agglomerations are divided based on the urban daily-communication-areas. The study found that: ①The level of urban time accessibility is divided into seven levels, showing a center-periphery ring structure in space. The central region expands along the axis of railway trunk lines and high-speed railway lines and the corridor effect is obvious. The eastern region of urban accessibility is superior to the western region. ②The city pairs with time accessibility less than 2 hours constitute a “Five vertical and five horizontal” zonation pattern connected by Beijing-Guangzhou, Beijing-Harbin, Beijing-Shanghai, Beijing-Fuzhou, Hangzhou-Fuzhou-Shenzhen, Qingdao-Taiyuan, Xuzhou-Lanzhou, Shanghai-Wuhan-Chengdu, Shanghai-Kunming and Guangzhou-Kunming high-speed railway lines. Cities with time accessibility less than 10 hours cover most of the southeast part of the “Huhuan-yong line” and the urban belt is transformed into the urban network. Cities with time accessibility longer than 10 hours are mainly connected to the eastern and western regions with a long spatial distance.③ The daily-communication-areas take 31 cities as the center and expand from small to large in accordance with the time accessibility. The non-overlapping daily-communication-areas cover a total of 227 administrative units. Based on the daily-communication-areas, this paper divides 19 urban agglomerations and Lasa urban circle, providing a reference way for the division of urban agglomerations.