705 / 2019-05-02 03:37:13

Characterization of the Arabidopsis tRNAome during Pattern Triggered Immunity

tRNA,PTI,mRNA decay,codon usage

全体主题 > RNA Biology

Previous findings have indicated that plants rapidly accumulate high levels of both charged and uncharged forms of specific tRNAs in response to pathogen recognition by cell surface localized Pattern Recognition Receptors. In addition, we have identified a pattern of differential codon usage in pathogen induced transcripts. Recently, novel approaches applied in yeast and mammalian systems have successfully overcome earlier problems associated with Next-gen sequencing of highly structured tRNAs. These advances presented the opportunity to adapt methods in order to perform the first ever high-resolution analysis of a plant “tRNAome” as a means of further exploring the function of differential accumulation of specific tRNAs in plant immune responses. We are examining the abundance of tRNA during the induced immune response in Arabidopsis through a combination of biochemical and next-generation sequencing techniques. This is being done under conditions identical to genome-wide transcriptional and translational studies from our laboratory, allowing for comparative analysis of output from multiple genetic mechanisms. Preliminary results suggest that changes in codon optimality resulting from coordinated modification of tRNA supply (isoacceptor abundance) and demand (codon usage), may facilitate rapid global changes in gene expression. This work is anticipated to provide a comprehensive analysis of the dynamics and function of tRNA in rapid plant immune responses.