681 / 2019-05-01 00:03:31

Transcriptome analysis revealed the underlying mechanism of wheat root response to different nitrogen levels

RNA-Seq,Wheat,Nitrogen level,Root

全体主题 > Nutrition

Roots play a decisive role in nitrogen (N) absorption. In this study, wheat cv Spitfire, was treated with three concentrations of ammonium nitrate based on the field practical research. In order to uncover the mechanism of wheat root formation that influenced by N levels from the transcriptional level, RNA-Seq was carried out. 16128 DEGs detected were then clustered by STEM analysis into eight expression profiles. DEGs with continuously negative response, persistently negative response and persistently positive response along the increasing of N level were significantly clustered. As GO analysis showed, “Plasma membrane (GO: 0005886)” was common in the three profiles. It indicated that DEGs expressed on membrane may act as diverse biological process and molecular function in response to changes of nitrogen level. Interestingly, through KEGG enrichment analysis, pathways involving in transporters (ko02000) were detected to be the most significantly enriched pathways, which echoed the result GO analysis. Also, the signaling pathways including plant hormones and transcription factors and the relationship between carbon-nitrogen metabolisms played important roles in the response of wheat roots to different nitrogen levels.
These results obtained are beneficial to a better understanding of the potential molecular and cellular mechanisms regulating root morphology changes under different N levels, and contributing to more reasonable soil fertility management measures.