660 / 2019-04-30 10:41:31

Molecular cloning and expression analysis of MADS-box gene GBM6 Ginkgo biloba

Ginkgo biloba, MADS-box, Cloning, Expression analysis

Growth and Development > Growth and Development

The MADS-box transcription factors belong to a conservative gene family, the MADS-box gene family plays an important role in plant growth and development. Here, we isolated and characterized a TM3/SOC1-like gene Gb01884 from the shoot apical meristem (STM) of Ginkgo biloba. Then the fragment of Gb01884 was cloned by RT-PCR and the sequence aligment was performed through NCBI platform. The alignment of TM3/SOC1-like genes in different species revealed the presence of a highly conserved region designated as SOC1 motif in the C-terminal of TM3/SOC1 protein sequences,so we named it GBM6. Phylogenetic analysis indicated that GBM6 belongs to the MIKC-type MADS-box gene family and highly homologous to the MADS-box genes from Gnetum Gnemon, Picea Abies and Pinus Radiata, while showed relatively low homologous to the MADS-box gene from Arabidopsis Thaliana.The expression of GBM6 was detected in both vegetative and reproductive organs. The expression levels of GBM6 was significantly higher in leaves than that in SAMs, female flowers and male-flowers . Furthermore, the expression level of GBM6 gradually decrease along with the process of flower bud differentiation and the decreasing magnitude was much higher in leaves than those in other tissues. This expression pattern is similar to those of reported TM3/SOC1 family genes in other species. Therefore, we speculated that GBM6 might play a role in regulating flower initiation and floral development in Ginkgo biloba.