659 / 2019-04-30 10:06:27

Identification of the MADS-box Gene Family and a GBM5 in Ginkgo biloba

Ginkgo biloba, MADS-box gene family, GBM5, Phylogenetic analysis, Expression analysis

Growth and Development > Growth and Development

MADS-box genes are important transcription factors for plant development, especially for determining the initiation of flowering and floral organogenesis. In this study, we performed a systemic analysis including transcriptome characterization, classification, and expression of the MADS-box transcription factor gene family. Among the MADS-box gene family, GBM5 , a floral-specific gene, was isolated and characterized as a homologous of the AGAMOUS genes. The GBM5 gene was then cloned and its expression patterns were studied in this study. In this study, 15 Ginkgo biloba MADS-box genes were identified according to the transcriptome data. The fragment of GBM5 was obtained by RT-PCR from floral buds of Ginkgo biloba. The open reading frame of GBM5 is 732 bp, which encodes a 236-amino acids protein product. The phylogenetic analysis indicated that GBM5 belongs to the AGMOUS group of MADS-box proteins. qRT-PCR analysis showed that the expression of GBM5 gene has a significant downward trend, with the flower bud differentiation process. We proposed that GBM5 gene might play an important role in the flowering regulation of Ginkgo biloba.15 MADS-box genes were identified according to the Ginkgo biloba transcriptome data, and among them a GBM5 gene was cloned. This study laid the foundation for the further screening of MADS-box genes and consequent functional analysis in Ginkgo biloba.