645 / 2019-04-29 10:51:30

A New Heat Response Factor 1 (NHRF1) is involved in alternative splicing and heat stress response in Arabidopsis thaliana

Arabidopsis; alternative splicing; heat stress; salicylic acid

全体主题 > Abiotic Stress

Alternative splicing (AS), an important modulator of gene expression, can increase proteome diversity and regulate mRNA levels by generating multiple transcripts from the same gene. In plants, this post-transcriptional mechanism is noticeably induced by environmental stimuli and plays an important role in the regulation of gene expression for biotic/abiotic stress responses. Here we found that a New Heat Response Factor 1 (NHRF1) was alternatively spliced to multiple isoforms. At least, there are four splicing patterns encoding four kinds of proteins: NHRF1.1, NHRF1.2, NHRF1.3, and NHRF1.4. The transcriptional levels of splicing patterns of NHRF1 from leaves were sharping altered when plants exposed to heat condition. Further analysis showed that transcription level of NHRF1.1 was accumulated in larger quantity under heat stress, whereas NHRF1.2 was almost completely suppressed after one hour. We also quantified the translation for each isoforms in time-course under heat stress regarding their mRNA level using western blotting in vivo. Furthermore, overexpression plants with different isoforms revealed that some of them played essential role in heat tolerance in plants through moderating salicylic acid (SA) metabolism. Collectively, this work will uncover the accurate splicing patterns for NHRF1 in response to heat stress and illustrate the biological importance of them in heat stress tolerance for Arabidopsis.