641 / 2019-04-28 21:31:38

Identification and Functional Study of IDD Family Genes Related to the SHORT-ROOT and SCARECROW Developmental Pathway

Stem cell renewal; IDD family proteins； expression patterns; multiple mutants; transcriptomic; ChIP-seq

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Continuous root growth relies on the maintenance of stem cells in the root apical meristem. However, the molecular mechanism of stem cell renewal remains unclear.
SHORT-ROOT (SHR) and SCARECROW (SCR) are important factors controlling stem cell renewal and ground tissue patterning in the Arabidopsis root. Although SHR and SCR regulate gene directly, they do not bind to DNA, rather, they control gene expression through the IDD family proteins. However, no single idd mutant recapitulates the short root phenotype of the shr or scr mutant, which suggests that IDDs may function redundantly in stem cell maintenance. Through a systematic analysis of the expression patterns of IDD genes using promoter::GUS reporter transgenic lines, we found several IDDs expressed in root stem cells and the ground tissue. Supporting a role for these IDD proteins in the SHR and SCR developmental pathway, we showed by yeast two-hybrid method that they physically interact with SHR and SCR. Next, we will further confirm these interactions by BiFC and Co-IP. We have also created multiple mutants for these IDD genes for functional analyses. Finally, downstream genes of these IDD genes will be identified through transcriptomic analyses and ChIP-seq, which will be used to construct the regulatory network underlying root stem cell maintenance. These experiments will deepen our understanding of root developmental mechanisms.