617 / 2019-04-24 10:02:07

RHS10-mediated inhibition of Arabidopsis root hair growth

Arabidopsis thaliana,root hair,RHS10,Cell wall signal

Growth and Development > Growth and Development

Plant cell growth is restricted by the cell wall, and the cell-wall dynamics would play as signal for cytoplasmic and nuclear events for cell growth. Among diverse receptor kinases, ROOT HAIR SPECIFIC 10 (RHS10) belongs to the receptor kinase subfamily with the Pro-rich extracellular domain. RHS10 defines the root hair length of Arabidopsis thaliana by negatively regulating hair growth and modulates the duration of root hair growth rather than the growth rate. RHS10 showed strong association with the cell wall most likely through its extracellular Pro-rich domain (ECD). The cytoplasmic kinase domain of RHS10 showed autophosphorylation. RHS10 suppressed the accumulation of reactive oxygen species (ROS) in the root which is necessary for root hair growth. RHS10 is likely to inhibit root hair growth downstream of hormone signaling because auxin and ethylene precursor could not restore RHS10-inhibited hair growth. To characterize the signaling mechanism for RHS10-mediated root hair inhibition, three approaches have been taken; transcriptome analysis to find RHS10 target genes, phosphoproteomic approach to identify RHS10 substrates, and genetic screening for RHS10 signaling components. These approaches would illustrate how RHS10 transduces the cell wall signal to modulate root hair growth.