606 / 2019-04-22 18:19:43

Studies on the Molecular Mechanism of the Interaction between ABI5 and AFP4

AFP4,ABI5,transcriptional activity,phosphorylated,Interaction

全体主题 > Signal Transduction

ABI5, an important transcription factor in ABA signal transduction, participates in seed germinate, seed dormancy, flowering, and various biotic or abiotic stresses. Four proteins had been found to be interacting with ABI5 in Arabidopsis until now. To understand the physiological roles and molecular mechanisms of these interacting proteins, one of them, AFP4, was investigated in yeast system. ABI5 or AFP4 has no transcriptional activity in yeast cells when they were expressed alone. However, ABI5 presented transcriptional activity while it was co-expressed with AFP4. AFP4 interacts only with the amino-portion of ABI5. Further deletion analyses showed that AFP4 bound to the potential phosphorylated sites of ABI5. The portion of the AFP4 fragment from 231 to 319 residues can interact with ABI5, but no interaction from the 1 to 230 residues. Moreover, the interaction of the 231 to 319 fragment with ABI5 is stronger than the full length of AFP4. Nonetheless, the positions of AFP4 interacted with ABI5 seems to be more than one according to the two-way deletion experiments of AFP4.