531 / 2019-03-06 16:33:11

Mapping QTLs for 15 morpho-metric traits in Arabidopsis thaliana using Col-0 × Don-0 population

Arabidopsis, phenotyping, mapping population, Single nucleotide polymorphism (SNP), QTL mapping

From models to crops > From models to crops

Genome wide QTL mapping was conducted in Arabidopsis thaliana using F2 mapping population (Col-0 × Don-0) and SNPs markers. Total five linkage groups were obtained with number of SNPs per linkage group varied from 45 to 59. Single locus analysis detected a total of 36 QTLs for 15 traits with number of QTLs per trait ranging from one (root length, root dry biomass, cauline leaf width, number of internode and internode distance) to seven (for bolting days). Phenotypic variance explained (PVE) and logarithm of the odds ratio (LOD) of these 36 QTLs found upto 38.17% and 6.26 respectively. Further, two locus analysis identified one main effect QTL (M-QTL) and four epistatic QTLs (E-QTLs). Five major QTLs (PVE >15.0%) may found to be useful for fine mapping to identify genes associated with respective traits, and also for development of specialized population through marker assisted selection. The identification of additive and dominant effect QTLs and desirable alleles of each of above mentioned traits would also be important for future research.