530 / 2019-03-05 16:55:10

A cell-culture based system for high throughput chemical perturbation studies of cell division orientation

cell culture, chemical biology, cell division orientation

全体主题 > Cell Biology

Due to their sessile nature and the presence of rigid cell walls, plants need to rely on
highly regulated oriented cell divisions to control their final three-dimensional shape.
The intrinsic, generic machineries that execute mitosis and cytokinesis are relatively
well understood, however, the mechanism that governs cell division plane
orientation is a major unresolved question in plant biology. We here set up a cell
culture-based system that can be used for high throughput chemical perturbation
studies of the cell division orientation event. Combining this cell base color reporter
system with a high throughput automated confocal imaging system, we screened
15.040 compounds for a possible effect on cell division orientation. We successfully
identified and confirmed 52 compounds from this primary screen. We next
performed a secondary screen using Arabidopsis thaliana primary root meristems
and were able to identify 5 molecules that showed a reproducible effect on cell
division orientation in plant roots. These novel chemicals affecting cell division
orientation will be useful, reversible tools for studying division plane orientation in a
various set of plants.