521 / 2019-03-02 07:05:33

Extracellular ATP as a danger signal in Arabidopsis

receptor, plasma membrane, stress, pamp, mamp

全体主题 > Plant Microbe Interactions & Microbiomes

Past research has shown that extracellular ATP (eATP) acts as a danger associated molecular pattern (DAMP). eATP is released from plants as a result of elicitation or tissue damage, resulting in elevated ROS production, elevation of cytoplasmic calcium, NO production, and other plant stress responses. Past work identified a lectin-receptor kinase, P2K1 (DORN1), as the primary eATP receptor in Arabidopsis (Choi et al., 2014). Subsequent work showed that P2K1 can directly phosphorylate NADPH oxidase (RBOHD) resulting in increased ROS production (Chen et al., 2017). The identification of RBOHD as a substrate was the result of mass spectrometric screening for peptides phosphorylated by P2K1. This screen yielded ~30 putative P2K1 kinase substrates of which we have been able to confirm at least half using a variety of in vitro and in vivo assays. Among these P2K1 substrates are enzymes that modify receptor-like kinases, as well as those involved in important metabolic pathways. The presentation will cover our current information regarding the complexity of eATP signaling in plants and what general principles are being revealed by studying this specific DAMP receptor.

Choi et al. (2014) Identification of a plant receptor for extracellular ATP. Science 343: 290-294.
Chen et al. (2017) Extracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture. Nature Commun. 8: 2265.