510 / 2019-03-01 10:38:53

Imaging sugar distribution in ABA-treated seeds using FRET sugar sensors

ABA,sugar,FRET sugar sensor

全体主题 > Abiotic Stress

Seed germination is a complex process, which is controlled by intrinsic and environmental cues. Some key events required for the completion of seed germination at the cellular and tissue level have not been identified, especially in the presence of environmental stresses. Hormones, including abscisic acid (ABA), have a significant effect on the regulation of seed germination. ABA metabolism and signaling pathways are altered when seeds are subjected to environmental changes or stresses. It has been well-established that ABA inhibits seed germination, which can be overcome by exogenous sugar. We are interested in understanding how ABA affects sugar remobilization at the cellular level. We use a Förster Resonance Energy Transfer (FRET) sensor to monitor sugar distribution during seed germination with or without ABA-treatment. Intriguingly, we found that the level of glucose was dramatically reduced in the hypocotyl region in ABA-treated seeds compared to control seeds. We continue to explore the underlying mechanism causing this to occur.