470 / 2019-02-27 15:44:27

Molecular Mechanism of ATG9 vesicle in Arabidopsis thialiana

autophagosome,membrane trafficking,endosome,autohpagy,atg9 vesicle

全体主题 > Cell Biology

Macroautophagy (here after as autophagy) functions as a fundamental metabolism process to clear up the damaged or excessive proteins/cellular structures, so that the cellular homeostasis can be maintained for cell development and growth under stress conditions. A double membrane structure named autophagosome will sequester the cargoes and deliver them into the vacuole for degradation or recycling. The core AuTophaGy-related (ATG) proteins for autophagosome biogenesis are highly conserved in plant genome. Recent advances in plant autophagy research highlight several plant-specific regulators for autophagosome biogenesis, some of which appear to participate in autophagy regulation under specific stress conditions.ATG9 is the only transmembrane protein in the core ATG machinery, and resides on mobile compartments, termed ATG9 vesicles, which have been shown to play a crucial role during autophagy. Recently, we have demonstrated that in contrast to yeast and mammalian cells, Arabidopsis ATG9 plays a distinct role in autophagosomal outgrowth from the ER. In this study, we aim to characterize the nature of ATG9 vesicle and its molecular mechanism in plant autophagy using a combination of cellular, molecular, biochemical and genetic approaches.