444 / 2019-02-14 22:22:04

The Novel Functions of Plant Bro1 Domain Proteins

ESCRT; Bro1 Domain; Vacuolar Protein; Multivesicular Body/Prevacuolar Compartment (MVB/PVC)

全体主题 > Cell Biology

The Bro1 domain occurs in a number of eukaryotic proteins. In the Arabidopsis genome, there are five genes that encode a Bro1-domain. AtBRO1/ALIX corresponds to a conserved multifunctional class of proteins, which functions to recruit the de-ubiquitinating enzyme to remove ubiquitin from cargo and also selectively sorts ubiquitinated cargo at an early step in endocytosis [1]. However, the function of other Bro1-domain proteins remains unknown. Here we report on the role of a plant-specific Bro1-domain protein BRAF, whose function as a unique evolutionary ESCRT negative regulator in orchestrating intraluminal vesicle formation in multivesicular body/prevacuolar compartment (MVB/PVC) and the sorting of membrane proteins for degradation in plants [2]. Supported by grants from ZAFU Starting Funding (2034020140), and the 111 Project (D18008) to J.S.; Research Grants Council of Hong Kong (CUHK14130716, 14102417, and AoE/M-05/12), the National Natural Science Foundation of China (91854201 and 31670179), and CUHK Research Committee to L.J.
References:
[1] Shen, J., Gao, C., Zhao, Q., Lin, Y., Wang, X., Zhuang, X., and Jiang, L. (2016). AtBRO1 Functions in ESCRT-I Complex to Regulate Multivesicular Body Protein Sorting. Mol. Plant 9, 760–763.
[2] Shen, J., Zhao, Q., Wang, X., Gao, C., Zhu, Y., Zeng, Y., and Jiang, L. (2018). A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis. Nat Commun 9, 3784.