404 / 2019-02-23 16:31:41

Sequestration of a Transposon-Derived siRNA by a Target Mimic Imprinted Gene Induces Postzygotic Reproductive Isolation in Arabidopsis

transposable elements,siRNAs,target mimic,hybridization barrier,endosperm,ploidy

全体主题 > Epigenetics

Genomic imprinting is an epigenetic phenomenon occurring in mammals and flowering plants, causing
genes to be expressed depending on their parent of origin. In plants, genomic imprinting is mainly confined to the endosperm, a nutritive tissue supporting embryo growth, similar to the placenta in mammals. Here, we show that the paternally expressed imprinted gene PEG2 transcript sequesters the transposable element (TE)-derived small interfering RNA (siRNA) siRNA854 in the endosperm. siRNA854 is
present in the vegetative cell of pollen and transferred to the central cell of the female gametophyte after
fertilization, where it is captured by PEG2. Depletion of siRNA854 as a consequence of increased PEG2
transcript levels establishes a reproductive barrier and prevents successful hybridizations between
plants differing in chromosome number (ploidy). Thus, the balance of a male gamete accumulating TE-derived siRNA and a paternally expressed imprinted gene regulate triploid seed viability, revealing a transgenerational speciation mechanism.