401 / 2019-02-23 05:44:31

RNA Polymerase II-Independent Recruitment of SPT6L at Transcription Start Sites in Arabidopsis

RNA Polymerase II-Independent Recruitment of SPT6L at Transcription Start Sites in Arabidopsis

全体主题 > RNA Biology

SPT6 is a conserved elongation factor that is associated with phosphorylated RNA polymerase II (RNAPII) during transcription. Recent transcriptome analysis in yeast mutants show its potential role in the control of transcription initiation at genic promoters, but, how this is achieved and linked to elongation remains to be elucidated. Here we first present the genome-wide occupancy of Arabidopsis SPT6-like (SPT6L) and demonstrate its conserved role in facilitating RNAPII occupancy across transcribed genes. Further, we show that SPT6L enrichment is shifted, unexpectedly, from gene body to transcription starting site (TSS) when its association with RNAPII is disrupted. Protein domains required for proper function and enrichment of SPT6L on chromatin are subsequently identified. Finally, we demonstrate that recruitment of SPT6L at TSS is indispensable for its spreading along gene body during transcription. These findings refine the mechanisms underlying SPT6L recruitment in transcription and shed light on the coordination between transcription initiation and elongation