462 / 2018-09-05 12:14:44

The effects of substrate structural factors on high solids enzymatic saccharification of alkali-pretreated sugarcane bagasse

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Filter paper and xylan were selected as model substrates simulating cellulose and hemicellulose components. Enzymatic hydrolysis of different substrates: pure filter paper, xylan added system, alkali pretreated sugarcane bagasse (SCB) were studied at varying solids loading to assess the effect of hemicellulose on substrate conversion at high solids loading. Meanwhile, hydrophilic lignin i.e. sulfonated lignin (SL) and alkali lignin (AL) was added to 15% alkali-pretreated SCB hydrolysis to investigate lignin influence on cellulose digestibility. It was found that xylan to be as the physical barrier had inhibition effect on cellulose hydrolysis. Enzymatic saccharification of alkali pretreated SCB were significantly improved by supplementing with SL and AL, the effect of lignin on biomass conversion was depending not only on the hydrophilic characteristics of lignin, but also on the lignin type presented in different substrates.