China is a big agricultural country, the treatment of huge amounts of herbaceous biomass such as rice husks faces serious environmental challenges. The biomass co-firing with pulverized coal for power generation is considered to be an effective solution to this problem. Due to the significant differences between the combustion of biomass and coal, the flame instability occurs easily if amount of the biomass is added into the coal. So, the ratio of biomass in the mixture fuel is limited. In this paper, an O2-enriched method is proposed to increase the proportion of biomass and improve the stability of co-combustion. Therefore, the ignition characteristics of biomass under O2-enriched atmosphere is the basic research content of the biomass and fuel combustion. The ignition characteristics of a single herbaceous biomass particle with particle sizes of 0.5-0.7mm in the mole fraction range of O2 from 21% to 70% in N2 were studied in this work. The herbaceous biomass particles were burned in a drop tube furnace setting at 1273K, and a high-speed camera in the 1000-shots-a-second mode was used to record the images of the herbaceous biomass particle ignition process. The radiant energy analysis technology is also used to analysis the ignition mechanism of the herbaceous biomass particle. The results show that the ignition mechanism and ignition location of the herbaceous biomass particle is strongly depend with the O2 concentration, and the enhancement of the O2 concentration significantly improve the combustion performance of the biomass.