1 / 2018-08-26 21:45:05

Adhesion Activity Assay of Lactobacillus plantarum AKK 30 Combined with Oligosaccharides

bacterial adhesions, Lactobacillus plantarum, inulin oligosaccharides, MOS

Adhesion Activity Assay of Lactobacillus plantarum AKK 30 Combined with Oligosaccharides
Alfi Sophian 1.3 Hardi Julendra2.3, Ahmad Sofyan 2, Karimi 2 , Abinawanto3*
1. National Agency of Drug and Food Control in Gorontalo
2. Research Unit Natural Product Technology- Indonesian Institute of Sciences (LIPI), Yogjakarta
3. Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA) – the University of Indonesian (UI), Depok, Jakarta.

*Corresponding author: abinawanto.ms@sci.ui.ac.id

Abstract

This objective of study was to measure the activity of lactic acid bacteria (LAB) adhesions in the intestinal mucosa. Those of lactic acid bacteria used were L. plantarum AKK 30 and were combined either with inulin oligosaccharides (OS) or mannan oligosaccharides (MOS). The oligosaccharides concentration used in this experiment were, 0%, 0.5%, 1%, 1.5% and 2%, respectively. Data collected were total plate count (TPC) before and after adhesion assay. The ultra structure of L. plantarum AKK 30 was observed by scanning electro microscope (SEM). The TPC results of the combination of L. plantarum and inulin (OS) were 18 x 107(0%), 48 x 107 (0.5%), 109 x 107 (1.0%), 129 x 107 (1.5%), and 99 x 107 (2.0%), respectively, while the combination of L. plantarum and MOS were 18 x 107 (0%), 53 x 107 (0.5%), 125 x 107 (1.0%), 210 x 107 (1.5%), and 66 x 107 (2.0%), respectively. The highest growth average of LAB were shown by the combination L. plantarum and 1.5% of MOS. In addition, the TPC results of assay adhesion showed that the combination of L. plantarum and inulin oligosaccharides were: 3.89 x 108 (0.5%), 4.39x 108 (1.0%), 3.19 x 108 (1.5%), and 5.09 x 108 (2.0%), respectively, whereas the combination of L. plantarum and MOS were: 7.29 x 108 (0.5%), 15.49 x 108 (1.0%), 2.89 x 108 (1.5%), and 4.49 x 108 (2.0%), respectively. There were significant different in the TPC results of assay adhesion among the treatment groups and control (1.16 x 108) . The highest activity of LAB adhesion was shown by the combination of L. plantarum and 1% of MOS. The TPC result was supported by the SEM observation. The combination of L. plantarum with oligosaccharides affects the growth and the adhesion length of lactic acid bacteria in the intestine. Further, the optimum concentration was shown by 1.0% of MOS combined with L. plantarum.
Keywords: bacterial adhesions, Lactobacillus plantarum, inulin oligosaccharides, MOS