74 / 2018-07-31 17:43:50

Printing liver cell scaffold for liver function recovery with stereolithography

DLP,stereolithography,cell scaffold,liver

全体主题 > iii. Engineering tissues and organs in vitro and in vivo

The liver cancer leads to the highest morbidity among all kinds of cancers in the world. Due to the shortage of liver for transplantation, surgical resection is still the most effective method for the treatment of various benign and malignant liver tumours. However, postoperative liver failure has been the most serious complication of most patients with liver resection.
In this study, we developed a scaffold for the construction of an artificial liver support system, which can replace the patient’s impaired liver. Here, GelMA is used as a scaffold material, and ECM is added as an auxiliary material, which can provide the necessary cell survival environment for cells in the liver scaffold. The cell-laden bioink was developed by adding hiHep cells into GelMA/ECM hydrogels. The hiHep cells are mature liver cells, but they have good proliferative capacity and can ensure good proliferation ability under the premise of ensuring the function of mature liver cells.
A DLP (Digital Light Processing)-based stereolithographic technology was applied to fabricate the artificial liver scaffold, and the activity and survival of the cells in the scaffold are regulated by adjusting the fabrication conditions. This work provides an artificial liver scaffold to solve liver failure for liver function recovery and regeneration.